Real-time imaging of VCAM-1 mRNA in TNF-α activated retinal microvascular endothelial cells using antisense hairpin-DNA functionalized gold nanoparticles.

Uddin MI, Jayagopal A, Wong A, McCollum GW, Wright DW, Penn JS
Nanomedicine. 2018 14 (1): 63-71

PMID: 28890107 · PMCID: PMC5742066 · DOI:10.1016/j.nano.2017.08.018

Vascular cell adhesion molecule 1 (VCAM-1) is an important inflammatory biomarker correlating with retinal disease progression. Thus, detection of VCAM-1 mRNA expression levels at an early disease stage could be an important predictive biomarker to assess the risk of disease progression and monitoring treatment response. We have developed VCAM-1 targeted antisense hairpin DNA-functionalized gold nanoparticles (AS-VCAM-1 hAuNP) for the real time detection of VCAM-1 mRNA expression levels in retinal endothelial cells. The AS-VCAM-1 hAuNP fluorescence enhancement clearly visualized the TNF-α induced cellular VCAM-1 mRNA levels with high signal to noise ratios compared to normal serum treated cells. The scrambled hAuNP probes were minimally detectable under same image acquisition conditions. Intracellular hAuNPs were detected using transmission electron microscopy (TEM) analysis of the intact cells. In addition, the AS-VCAM-1 hAuNP probes exhibited no acute toxicity to the retinal microvascular endothelial cells as measured by live-dead assay.

Copyright © 2017 Elsevier Inc. All rights reserved.

MeSH Terms (14)

Animals Cells, Cultured Cell Survival DNA, Antisense Endothelium, Vascular Fluorescence Gold Metal Nanoparticles Mice Molecular Imaging Retinal Vessels RNA, Messenger Tumor Necrosis Factor-alpha Vascular Cell Adhesion Molecule-1

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