Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.

Snider CE, Willet AH, Chen JS, Arpağ G, Zanic M, Gould KL
J Cell Biol. 2017 216 (10): 3041-3050

PMID: 28784611 · PMCID: PMC5626552 · DOI:10.1083/jcb.201705070

Many eukaryotic cells divide by assembling and constricting an actin- and myosin-based contractile ring (CR) that is physically linked to the plasma membrane (PM). In this study, we report that cells lacking , which encodes a conserved PM scaffold for the phosphatidylinositol-4 kinase Stt4, build CRs that can slide away from the cell middle during anaphase in a myosin V-dependent manner. The Efr3-dependent CR-anchoring mechanism is distinct from previously reported pathways dependent on the Fes/CIP4 homology Bin-Amphiphysin-Rvs167 (F-BAR) protein Cdc15 and paxillin Pxl1. In , the concentrations of several membrane-binding proteins were reduced in the CR and/or on the PM. Our results suggest that proper PM lipid composition is important to stabilize the central position of the CR and resist myosin V-based forces to promote the fidelity of cell division.

© 2017 Snider et al.

MeSH Terms (8)

1-Phosphatidylinositol 4-Kinase Cell Cycle Proteins Cytokinesis Glycosylphosphatidylinositols GTP-Binding Proteins Myosin Type V Schizosaccharomyces Schizosaccharomyces pombe Proteins

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