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High-resolution hyperpolarized in vivo metabolic C spectroscopy at low magnetic field (48.7mT) following murine tail-vein injection.

Coffey AM, Feldman MA, Shchepin RV, Barskiy DA, Truong ML, Pham W, Chekmenev EY
J Magn Reson. 2017 281: 246-252

PMID: 28651245 · PMCID: PMC5544012 · DOI:10.1016/j.jmr.2017.06.009

High-resolution C NMR spectroscopy of hyperpolarized succinate-1-C-2,3-d is reported in vitro and in vivo using a clinical-scale, biplanar (80cm-gap) 48.7mT permanent magnet with a high homogeneity magnetic field. Non-localized C NMR spectra were recorded at 0.52MHz resonance frequency over the torso of a tumor-bearing mouse every 2s. Hyperpolarized C NMR signals with linewidths of ∼3Hz (corresponding to ∼6ppm) were recorded in vitro (2mL in a syringe) and in vivo (over a mouse torso). Comparison of the full width at half maximum (FWHM) for C NMR spectra acquired at 48.7mT and at 4.7T in a small-animal MRI scanner demonstrates a factor of ∼12 improvement for the C resonance linewidth attainable at 48.7mT compared to that at 4.7T in vitro. C hyperpolarized succinate-1-C resonance linewidths in vivo are at least one order of magnitude narrower at 48.7mT compared to those observed in high-field (≥3T) studies employing HP contrast agents. The demonstrated high-resolution C in vivo spectroscopy could be useful for high-sensitivity spectroscopic studies involving monitoring HP agent uptake or detecting metabolism using HP contrast agents with sufficiently large C chemical shift differences.

Copyright © 2017 Elsevier Inc. All rights reserved.

MeSH Terms (14)

Animals Carbon Isotopes Cell Line, Tumor Contrast Media Electromagnetic Fields Female Injections, Intravenous Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Mice Mice, Nude Neoplasms, Experimental Tail Veins

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