Evaluation of a novel fluorescent nanobeacon for targeted imaging of Thomsen-Friedenreich associated colorectal cancer.

Nakase H, Sakuma S, Fukuchi T, Yoshino T, Mohri K, Miyata K, Kumagai H, Hiwatari KI, Tsubaki K, Ikejima T, Tobita E, Zhu M, Wilson KJ, Washington K, Gore JC, Pham W
Int J Nanomedicine. 2017 12: 1747-1755

PMID: 28280339 · PMCID: PMC5340240 · DOI:10.2147/IJN.S124174

The Thomsen-Friedenreich (TF) antigen represents a prognostic biomarker of colorectal carcinoma. Here, using a nanobeacon, the surface of which was fabricated with peanut agglutinin as TF-binding molecules, we demonstrate that the nanobeacon is able to detect TF antigen in frozen and freshly biopsied polyps using fluorescence microscopy. Our results provide important clues about how to detect aberrant colonic tissues in the most timely fashion. Given the versatile application method for this topical nanobeacon, the protocol used in this work is amenable to clinical colonoscopy. Moreover, the prospects of clinical translation of this technology are evident.

MeSH Terms (11)

Adenocarcinoma Adenoma Antigens, Tumor-Associated, Carbohydrate Colorectal Neoplasms Fluorescent Dyes Humans Microscopy, Fluorescence Molecular Probes Nanoparticles Optical Imaging Peanut Agglutinin

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