Transcription from the stromelysin promoter is induced by interleukin-1 and repressed by dexamethasone.

Frisch SM, Ruley HE
J Biol Chem. 1987 262 (34): 16300-4

PMID: 2824488

The stromelysin gene encodes a potent tissue-degrading proteinase whose activity is important in tissue-remoldeling processes such as wound healing, the inflammatory reaction, rheumatoid arthritis, tumor invasion, and possibly embryonic development. In light of the ability of interleukin-1 to amplify, and ability of glucocorticoids to attenuate the inflammatory response, we tested interleukin-1 and dexamethasone for regulatory effects on stromelysin gene expression. We report that interleukin-1 induces the stromelysin gene, and dexamethasone diminishes the level of induction by interleukin-1, epidermal growth factor, phorbol ester, and cAMP elevation (elicited by cholera toxin). Similar responses are conferred upon a chloramphenicol acetyltransferase coding sequence by a 700-base pair stromelysin 5'-flanking fragment, implying transcription regulation by sequence elements in this region.

MeSH Terms (19)

Animals Arthritis, Rheumatoid Base Sequence Cholera Toxin Cyclic AMP Dexamethasone Epidermal Growth Factor Gene Expression Regulation Interleukin-1 Matrix Metalloproteinase 3 Metalloendopeptidases Molecular Sequence Data Neoplasm Invasiveness Promoter Regions, Genetic Rabbits Repetitive Sequences, Nucleic Acid Tetradecanoylphorbol Acetate Transcription, Genetic Wound Healing

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