Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas.

Wang YJ, Golson ML, Schug J, Traum D, Liu C, Vivek K, Dorrell C, Naji A, Powers AC, Chang KM, Grompe M, Kaestner KH
Cell Metab. 2016 24 (4): 616-626

PMID: 27732837 · PMCID: PMC5123805 · DOI:10.1016/j.cmet.2016.09.007

The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High-dimensional analysis of our marker protein expression revealed three major clusters of beta cells within individuals. Proliferating beta cells are confined to two of the clusters.

Copyright © 2016 Elsevier Inc. All rights reserved.

MeSH Terms (7)

Cell Aggregation Cell Proliferation Flow Cytometry Harmine Humans Islets of Langerhans Single-Cell Analysis

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