Fluorescence-based measurement of cystine uptake through xCT shows requirement for ROS detoxification in activated lymphocytes.

Siska PJ, Kim B, Ji X, Hoeksema MD, Massion PP, Beckermann KE, Wu J, Chi JT, Hong J, Rathmell JC
J Immunol Methods. 2016 438: 51-58

PMID: 27594594 · PMCID: PMC5065394 · DOI:10.1016/j.jim.2016.08.013

MeSH Terms (18)

Amino Acid Transport System y+ B-Lymphocytes Cell Line, Tumor Cellular Reprogramming Cystine Flow Cytometry Fluorescein-5-isothiocyanate Fluorescence Fluorescent Dyes Glutathione Humans Lymphocyte Activation Microscopy, Fluorescence Reactive Oxygen Species Receptors, Antigen, T-Cell Signal Transduction T-Lymphocytes Up-Regulation

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