An unbiased metric of antiproliferative drug effect in vitro.

Harris LA, Frick PL, Garbett SP, Hardeman KN, Paudel BB, Lopez CF, Quaranta V, Tyson DR
Nat Methods. 2016 13 (6): 497-500

PMID: 27135974 · PMCID: PMC4887341 · DOI:10.1038/nmeth.3852

In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current metrics of antiproliferative small molecule effect suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.

MeSH Terms (12)

Cell Line, Tumor Cell Proliferation Computer Simulation Dose-Response Relationship, Drug Drug Discovery Humans Microscopy, Fluorescence Models, Theoretical Molecular Biology Sensitivity and Specificity Small Molecule Libraries Time Factors

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