Neonatal colonization of mice with LGG promotes intestinal development and decreases susceptibility to colitis in adulthood.

Yan F, Liu L, Cao H, Moore DJ, Washington MK, Wang B, Peek RM, Acra SA, Polk DB
Mucosal Immunol. 2017 10 (1): 117-127

PMID: 27095077 · PMCID: PMC5073052 · DOI:10.1038/mi.2016.43

Development of the intestinal microbiota during early life serves as a key regulatory stage in establishing the host-microbial relationship. This symbiotic relationship contributes to developing host immunity and maintaining health throughout the life span. This study was to develop an approach to colonize conventionally raised mice with a model probiotic bacterium, Lactobacillus rhamnosus GG (LGG), and to determine the effects of LGG colonization on intestinal development and prevention of colitis in adulthood. LGG colonization in conventionally raised was established by administering LGG to pregnant mice starting at gestational day 18 and pups at postnatal days 1- 5. LGG colonization promoted bodyweight gain and increased diversity and richness of the colonic mucosa-associated microbiota before weaning. Intestinal epithelial cell proliferation, differentiation, tight junction formation, and mucosal IgA production were all significantly enhanced in LGG-colonized mice. Adult mice colonized with LGG showed increased IgA production and decreased susceptibility to intestinal injury and inflammation induced in the dextran sodium sulfate model of colitis. Thus, neonatal colonization of mice with LGG enhances intestinal functional maturation and IgA production and confers lifelong health consequences on protection from intestinal injury and inflammation. This strategy might be applied for benefiting health in the host.

MeSH Terms (20)

Animals Animals, Newborn Cell Proliferation Cells, Cultured Colitis Dextran Sulfate Disease Models, Animal Female Gastrointestinal Microbiome Humans Immunoglobulin A Intestinal Mucosa Intestines Lactobacillus rhamnosus Mice Mice, Inbred C57BL Pregnancy Probiotics Symbiosis Tight Junctions

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