Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons.

Meltzer S, Yadav S, Lee J, Soba P, Younger SH, Jin P, Zhang W, Parrish J, Jan LY, Jan YN
Neuron. 2016 89 (4): 741-55

PMID: 26853303 · PMCID: PMC4760923 · DOI:10.1016/j.neuron.2016.01.020

Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly.

Copyright © 2016 Elsevier Inc. All rights reserved.

MeSH Terms (22)

Animals Animals, Genetically Modified Cell Communication Dendrites Drosophila Drosophila Proteins Epidermis Focal Adhesion Kinase 1 Gene Expression Regulation, Developmental Green Fluorescent Proteins Immunoprecipitation Larva Mechanistic Target of Rapamycin Complex 2 Molecular Biology Multiprotein Complexes Mutation Nerve Tissue Proteins Receptors, Cell Surface Semaphorins Sensory Receptor Cells TOR Serine-Threonine Kinases Transfection

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