Regulation of a bifunctional mRNA results in synthesis of secreted and nuclear probasin.

Spence AM, Sheppard PC, Davie JR, Matuo Y, Nishi N, McKeehan WL, Dodd JG, Matusik RJ
Proc Natl Acad Sci U S A. 1989 86 (20): 7843-7

PMID: 2682630 · PMCID: PMC298167 · DOI:10.1073/pnas.86.20.7843

Probasin, a rat prostatic protein, is statistically related to members of a protein family that includes serum, cellular, and nuclear proteins. In vivo, probasin appears both in the secretions and in the nucleus of prostatic epithelial cells. Using primer extension and S1 nuclease protection assays we detected only one probasin mRNA. Thus, the localization of this protein to two separate cellular regions must be encoded by this one mRNA. Furthermore, in vitro translation of synthetic probasin mRNA demonstrated that a protein containing a signal peptide and a protein lacking a signal peptide were synthesized by initiation at different AUG codons. These data are consistent with a mechanism of translational regulation of a eukaryotic bifunctional mRNA.

MeSH Terms (17)

Amino Acid Sequence Androgen-Binding Protein Animals Base Sequence Cell Nucleus Cloning, Molecular Codon Genes Genes, Regulator Immunoenzyme Techniques Male Molecular Sequence Data Prostate Protein Biosynthesis Rats RNA, Messenger Sequence Homology, Nucleic Acid

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