Rat glucokinase gene: structure and regulation by insulin.

Magnuson MA, Andreone TL, Printz RL, Koch S, Granner DK
Proc Natl Acad Sci U S A. 1989 86 (13): 4838-42

PMID: 2662183 · PMCID: PMC297510 · DOI:10.1073/pnas.86.13.4838

The glucokinase gene is 15.5-kilobases long, appears to be present as a single copy, and contains 10 exons that range in size from 96 to 977 base pairs. The transcription start site was located 127 nucleotides upstream from the translation initiation codon. The 5' flanking DNA contains several regions similar to defined promoter elements. These include a probable "TATA box," an Sp1 binding site, and several elements related to liver-specific gene expression. In addition, we determined that transcription of the glucokinase gene increased at least 20-fold when diabetic rats were treated with insulin for 2 hr.

MeSH Terms (17)

Animals Base Sequence Cell Nucleus Diabetes Mellitus, Experimental Exons Gene Expression Regulation Genes Glucokinase Insulin Introns Liver Male Molecular Sequence Data Rats Rats, Inbred Strains Restriction Mapping Transcription, Genetic

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