Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.

Liv N, van Oosten Slingeland DS, Baudoin JP, Kruit P, Piston DW, Hoogenboom JP
ACS Nano. 2016 10 (1): 265-73

PMID: 26580231 · PMCID: PMC4729641 · DOI:10.1021/acsnano.5b03970

We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.

MeSH Terms (10)

Animals Bioreactors Cell Line Cells, Immobilized Chlorocebus aethiops Endocytosis Fibroblasts Microscopy, Electron, Scanning Optical Imaging Quantum Dots

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