Cordon bleu promotes the assembly of brush border microvilli.

Grega-Larson NE, Crawley SW, Erwin AL, Tyska MJ
Mol Biol Cell. 2015 26 (21): 3803-15

PMID: 26354418 · PMCID: PMC4626065 · DOI:10.1091/mbc.E15-06-0443

Microvilli are actin-based protrusions found on the surface of diverse cell types, where they amplify membrane area and mediate interactions with the external environment. In the intestinal tract, these protrusions play central roles in nutrient absorption and host defense and are therefore essential for maintaining homeostasis. However, the mechanisms controlling microvillar assembly remain poorly understood. Here we report that the multifunctional actin regulator cordon bleu (COBL) promotes the growth of brush border (BB) microvilli. COBL localizes to the base of BB microvilli via a mechanism that requires its proline-rich N-terminus. Knockdown and overexpression studies show that COBL is needed for BB assembly and sufficient to induce microvillar growth using a mechanism that requires functional WH2 domains. We also find that COBL acts downstream of the F-BAR protein syndapin-2, which drives COBL targeting to the apical domain. These results provide insight into a mechanism that regulates microvillar growth during epithelial differentiation and have significant implications for understanding the maintenance of intestinal homeostasis.

© 2015 Grega-Larson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

MeSH Terms (11)

Actin Cytoskeleton Actins Animals Cell Culture Techniques HEK293 Cells Humans Mice Microfilament Proteins Microvilli Protein Structure, Tertiary Syndecan-2

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