Cutting Edge: Redox Signaling Hypersensitivity Distinguishes Human Germinal Center B Cells.

Polikowsky HG, Wogsland CE, Diggins KE, Huse K, Irish JM
J Immunol. 2015 195 (4): 1364-1367

PMID: 26157177 · PMCID: PMC4530023 · DOI:10.4049/jimmunol.1500904

Differences in the quality of BCR signaling control key steps of B cell maturation and differentiation. Endogenously produced H2O2 is thought to fine tune the level of BCR signaling by reversibly inhibiting phosphatases. However, relatively little is known about how B cells at different stages sense and respond to such redox cues. In this study, we used phospho-specific flow cytometry and high-dimensional mass cytometry (CyTOF) to compare BCR signaling responses in mature human tonsillar B cells undergoing germinal center (GC) reactions. GC B cells, in contrast to mature naive B cells, memory B cells, and plasmablasts, were hypersensitive to a range of H2O2 concentrations and responded by phosphorylating SYK and other membrane-proximal BCR effectors in the absence of BCR engagement. These findings reveal that stage-specific redox responses distinguish human GC B cells.

Copyright © 2015 by The American Association of Immunologists, Inc.

MeSH Terms (14)

B-Lymphocytes B-Lymphocyte Subsets Cell Differentiation Gene Expression Germinal Center Humans Hydrogen Peroxide Immunophenotyping Oxidation-Reduction Palatine Tonsil Phenotype Protein Tyrosine Phosphatase, Non-Receptor Type 6 Receptors, Antigen, B-Cell Signal Transduction

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