Helicobacter pylori virulence factors affecting gastric proton pump expression and acid secretion.

Hammond CE, Beeson C, Suarez G, Peek RM, Backert S, Smolka AJ
Am J Physiol Gastrointest Liver Physiol. 2015 309 (3): G193-201

PMID: 26045613 · PMCID: PMC4525105 · DOI:10.1152/ajpgi.00099.2015

Acute Helicobacter pylori infection of gastric epithelial cells and human gastric biopsies represses H,K-ATPase α subunit (HKα) gene expression and inhibits acid secretion, causing transient hypochlorhydria and supporting gastric H. pylori colonization. Infection by H. pylori strains deficient in the cag pathogenicity island (cag PAI) genes cagL, cagE, or cagM, which do not transfer CagA into host cells or induce interleukin-8 secretion, does not inhibit HKα expression, nor does a cagA-deficient strain that induces IL-8. To test the hypothesis that virulence factors other than those mediating CagA translocation or IL-8 induction participate in HKα repression by activating NF-κB, AGS cells transfected with HKα promoter-Luc reporter constructs containing an intact or mutated NF-κB binding site were infected with wild-type H. pylori strain 7.13, isogenic mutants lacking cag PAI genes responsible for CagA translocation and/or IL-8 induction (cagA, cagζ, cagε, cagZ, and cagβ), or deficient in genes encoding two peptidoglycan hydrolases (slt and cagγ). H. pylori-induced AGS cell HKα promoter activities, translocated CagA, and IL-8 secretion were measured by luminometry, immunoblotting, and ELISA, respectively. Human gastric biopsy acid secretion was measured by microphysiometry. Taken together, the data showed that HKα repression is independent of IL-8 expression, and that CagA translocation together with H. pylori transglycosylases encoded by slt and cagγ participate in NF-κB-dependent HKα repression and acid inhibition. The findings are significant because H. pylori factors other than CagA and IL-8 secretion are now implicated in transient hypochlorhydria which facilitates gastric colonization and potential triggering of epithelial progression to neoplasia.

Copyright © 2015 the American Physiological Society.

MeSH Terms (17)

Achlorhydria Antigens, Bacterial Bacterial Proteins Cells, Cultured Epithelial Cells Gastric Acid Gastric Mucosa Helicobacter Infections Helicobacter pylori Humans Interleukin-8 NF-kappa B Promoter Regions, Genetic Proton Pumps Signal Transduction Sodium-Potassium-Exchanging ATPase Virulence Factors

Connections (1)

This publication is referenced by other Labnodes entities:

Links