Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints.

Columbus L, Kroncke B
Methods Enzymol. 2015 557: 329-48

PMID: 25950972 · DOI:10.1016/bs.mie.2014.12.005

Solution nuclear magnetic resonance structures of polytopic α-helical membrane proteins require additional restraints beyond the traditional Nuclear Overhauser Effect (NOE) restraints. Several methods have been developed and this review focuses on paramagnetic relaxation enhancement (PRE). Important aspects of spin labeling, PRE measurements, structure calculations, and structural quality are discussed.

© 2015 Elsevier Inc. All rights reserved.

MeSH Terms (8)

Animals Humans Models, Molecular Nitrogen Oxides Nuclear Magnetic Resonance, Biomolecular Proteins Protein Structure, Secondary Spin Labels

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