Effect of ACTH on the stability of mRNAs encoding bovine adrenocortical P-450scc, P-45011 beta, P-45017 alpha, P-450C21 and adrenodoxin.

Boggaram V, John ME, Simpson ER, Waterman MR
Biochem Biophys Res Commun. 1989 160 (3): 1227-32

PMID: 2543393 · DOI:10.1016/s0006-291x(89)80134-2

ACTH treatment of bovine adrenocortical cells in primary culture causes increased accumulation of mRNAs encoding cytochromes P-450SCC, P-450(11)beta, P-450(17) alpha, P-450C21 and adrenodoxin as well as increased transcriptional activity of their respective genes. In this study we have shown that ACTH does not greatly affect the half-life of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin. However, in the case of P-450SCC mRNA, ACTH causes a five-fold increase in the half-life leading to a significant stabilization of P-450SCC mRNA. Thus it appears that the levels of mRNAs encoding P-450(11)beta, P-450(17)alpha, P-450C21 and adrenodoxin are regulated by ACTH primarily at the transcriptional level, while that for P-450SCC is regulated at both the transcriptional and post-transcriptional levels.

MeSH Terms (14)

Adrenal Cortex Adrenocorticotropic Hormone Adrenodoxin Animals Cattle Cells, Cultured Cholesterol Side-Chain Cleavage Enzyme Cytochrome P-450 Enzyme System Drug Stability Gene Expression Regulation Half-Life Protein Biosynthesis RNA, Messenger Transcription, Genetic

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