The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast.

Ren L, Willet AH, Roberts-Galbraith RH, McDonald NA, Feoktistova A, Chen JS, Huang H, Guillen R, Boone C, Sidhu SS, Beckley JR, Gould KL
Mol Biol Cell. 2015 26 (2): 256-69

PMID: 25428987 · PMCID: PMC4294673 · DOI:10.1091/mbc.E14-10-1451

Schizosaccharomyces pombe cdc15 homology (PCH) family members participate in numerous biological processes, including cytokinesis, typically by bridging the plasma membrane via their F-BAR domains to the actin cytoskeleton. Two SH3 domain-containing PCH family members, Cdc15 and Imp2, play critical roles in S. pombe cytokinesis. Although both proteins localize to the contractile ring, with Cdc15 preceding Imp2, only cdc15 is an essential gene. Despite these distinct roles, the SH3 domains of Cdc15 and Imp2 cooperate in the essential process of recruiting other proteins to stabilize the contractile ring. To better understand the connectivity of this SH3 domain-based protein network at the CR and its function, we used a biochemical approach coupled to proteomics to identify additional proteins (Rgf3, Art1, Spa2, and Pos1) that are integrated into this network. Cell biological and genetic analyses of these SH3 partners implicate them in a range of activities that ensure the fidelity of cell division, including promoting cell wall metabolism and influencing cell morphogenesis.

© 2015 Ren et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (

MeSH Terms (21)

Amino Acid Sequence Cell Cycle Proteins Cell Division Cytokinesis Cytoskeletal Proteins Gene Regulatory Networks GTP-Binding Proteins Guanine Nucleotide Exchange Factors Immunoblotting Luminescent Proteins Microscopy, Confocal Molecular Sequence Data Mutation Protein Binding Proteome Proteomics Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid src Homology Domains Time-Lapse Imaging

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