Dyskerin, tRNA genes, and condensin tether pericentric chromatin to the spindle axis in mitosis.

Snider CE, Stephens AD, Kirkland JG, Hamdani O, Kamakaka RT, Bloom K
J Cell Biol. 2014 207 (2): 189-99

PMID: 25332162 · PMCID: PMC4210444 · DOI:10.1083/jcb.201405028

Condensin is enriched in the pericentromere of budding yeast chromosomes where it is constrained to the spindle axis in metaphase. Pericentric condensin contributes to chromatin compaction, resistance to microtubule-based spindle forces, and spindle length and variance regulation. Condensin is clustered along the spindle axis in a heterogeneous fashion. We demonstrate that pericentric enrichment of condensin is mediated by interactions with transfer ribonucleic acid (tRNA) genes and their regulatory factors. This recruitment is important for generating axial tension on the pericentromere and coordinating movement between pericentromeres from different chromosomes. The interaction between condensin and tRNA genes in the pericentromere reveals a feature of yeast centromeres that has profound implications for the function and evolution of mitotic segregation mechanisms.

© 2014 Snider et al.

MeSH Terms (14)

Adenosine Triphosphatases Centrosome Chromatin DNA-Binding Proteins Hydro-Lyases Kinetochores Microtubule-Associated Proteins Mitosis Multiprotein Complexes Ribonucleoproteins, Small Nuclear RNA, Transfer Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Spindle Apparatus

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