Production and characterization of monoclonal antibodies to the N-terminal domain of lambda repressor.

Breyer RM, Sauer RT
J Biol Chem. 1989 264 (22): 13348-54

PMID: 2526815

Monoclonal antibodies reactive with distinct regions of the N-terminal domain of the lambda repressor protein have been isolated. By comparing the affinities of these antibodies for mutant repressors with increased and decreased thermal stabilities, each of the antibodies can be shown to bind to epitopes accessible in the native conformation of the N-terminal domain. Experiments probing antibody binding to protein fragments, mutant variants, and peptides have also been used to define likely regions of contact between the antibodies and the N-terminal domain.

MeSH Terms (16)

Amino Acid Sequence Animals Antibodies, Monoclonal Antibodies, Viral Bacteriophage lambda Binding, Competitive Binding Sites, Antibody DNA-Binding Proteins Female Mice Mice, Inbred BALB C Protein Conformation Repressor Proteins Transcription Factors Viral Proteins Viral Regulatory and Accessory Proteins

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