The Nogo receptor NgR1 mediates infection by mammalian reovirus.

Konopka-Anstadt JL, Mainou BA, Sutherland DM, Sekine Y, Strittmatter SM, Dermody TS
Cell Host Microbe. 2014 15 (6): 681-91

PMID: 24922571 · PMCID: PMC4100558 · DOI:10.1016/j.chom.2014.05.010

Neurotropic viruses, including mammalian reovirus, must disseminate from an initial site of replication to the central nervous system (CNS), often binding multiple receptors to facilitate systemic spread. Reovirus engages junctional adhesion molecule A (JAM-A) to disseminate hematogenously. However, JAM-A is dispensable for reovirus replication in the CNS. We demonstrate that reovirus binds Nogo receptor NgR1, a leucine-rich repeat protein expressed in the CNS, to infect neurons. Expression of NgR1 confers reovirus binding and infection of nonsusceptible cells. Incubating reovirus virions with soluble NgR1 neutralizes infectivity. Blocking NgR1 on transfected cells or primary cortical neurons abrogates reovirus infection. Concordantly, reovirus infection is ablated in primary cortical neurons derived from NgR1 null mice. Reovirus virions bind to soluble JAM-A and NgR1, while infectious disassembly intermediates (ISVPs) bind only to JAM-A. These results suggest that reovirus uses different capsid components to bind distinct cell-surface molecules, engaging independent receptors to facilitate spread and tropism.

Copyright © 2014 Elsevier Inc. All rights reserved.

MeSH Terms (18)

Animals Capsid Proteins Cell Adhesion Molecules Cell Membrane CHO Cells Cricetulus GPI-Linked Proteins Host-Pathogen Interactions Humans Mice, Mutant Strains Myelin Proteins Neuraminidase Neurons Nogo Receptor 1 Receptors, Cell Surface Reoviridae Reoviridae Infections Virion

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