Implementation of a Gaussian beam laser and aspheric optics for high spatial resolution MALDI imaging MS.

Zavalin A, Yang J, Haase A, Holle A, Caprioli R
J Am Soc Mass Spectrom. 2014 25 (6): 1079-82

PMID: 24692046 · PMCID: PMC4035219 · DOI:10.1007/s13361-014-0872-5

We have investigated the use of a Gaussian beam laser for MALDI Imaging Mass Spectrometry to provide a precisely defined laser spot of 5 μm diameter on target using a commercial MALDI TOF instrument originally designed to produce a 20 μm diameter laser beam spot at its smallest setting. A Gaussian beam laser was installed in the instrument in combination with an aspheric focusing lens. This ion source produced sharp ion images at 5 μm spatial resolution with signals of high intensity as shown for images from thin tissue sections of mouse brain.

MeSH Terms (8)

Animals Cerebellum Lasers Mice Molecular Imaging Optics and Photonics Proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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