Combined scanning transmission electron microscopy tilt- and focal series.

Dahmen T, Baudoin JP, Lupini AR, K├╝bel C, Slusallek P, de Jonge N
Microsc Microanal. 2014 20 (2): 548-60

PMID: 24548618 · DOI:10.1017/S1431927614000075

In this study, a combined tilt- and focal series is proposed as a new recording scheme for high-angle annular dark-field scanning transmission electron microscopy (STEM) tomography. Three-dimensional (3D) data were acquired by mechanically tilting the specimen, and recording a through-focal series at each tilt direction. The sample was a whole-mount macrophage cell with embedded gold nanoparticles. The tilt-focal algebraic reconstruction technique (TF-ART) is introduced as a new algorithm to reconstruct tomograms from such combined tilt- and focal series. The feasibility of TF-ART was demonstrated by 3D reconstruction of the experimental 3D data. The results were compared with a conventional STEM tilt series of a similar sample. The combined tilt- and focal series led to smaller "missing wedge" artifacts, and a higher axial resolution than obtained for the STEM tilt series, thus improving on one of the main issues of tilt series-based electron tomography.

MeSH Terms (8)

Algorithms Artifacts Electron Microscope Tomography Imaging, Three-Dimensional Macrophages Microscopy, Electron, Scanning Transmission Models, Theoretical Nanoparticles

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