Jagged1 is essential for osteoblast development during maxillary ossification.

Hill CR, Yuasa M, Schoenecker J, Goudy SL
Bone. 2014 62: 10-21

PMID: 24491691 · PMCID: PMC4306467 · DOI:10.1016/j.bone.2014.01.019

Maxillary hypoplasia occurs due to insufficient maxillary intramembranous ossification, leading to poor dental occlusion, respiratory obstruction and cosmetic deformities. Conditional deletion of Jagged1 (Jag1) in cranial neural crest (CNC) cells using Wnt1-cre; Jagged1(f/f) (Jag1CKO) led to maxillary hypoplasia characterized by intrinsic differences in bone morphology and density using μCT evaluation. Jag1CKO maxillas revealed altered collagen deposition, delayed ossification, and reduced expression of early and late determinants of osteoblast development during maxillary ossification. In vitro bone cultures on Jag1CKO mouse embryonic maxillary mesenchymal (MEMM) cells demonstrated decreased mineralization that was also associated with diminished induction of osteoblast determinants. BMP receptor expression was dysregulated in the Jag1CKO MEMM cells suggesting that these cells were unable to respond to BMP-induced differentiation. JAG1-Fc rescued in vitro mineralization and osteoblast gene expression changes. These data suggest that JAG1 signaling in CNC-derived MEMM cells is required for osteoblast development and differentiation during maxillary ossification.

Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

MeSH Terms (23)

Animals Bone Density Bone Morphogenetic Proteins Calcification, Physiologic Calcium Calcium-Binding Proteins Cell Differentiation Embryo, Mammalian Intercellular Signaling Peptides and Proteins Jagged-1 Protein Maxilla Membrane Proteins Mesoderm Mice, Knockout Organ Size Osteoblasts Osteogenesis Palate Receptors, Fc Receptors, Notch Serrate-Jagged Proteins Signal Transduction X-Ray Microtomography

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