Production and auto-induction of transforming growth factor-alpha in human keratinocytes.

Coffey RJ, Derynck R, Wilcox JN, Bringman TS, Goustin AS, Moses HL, Pittelkow MR
Nature. 1987 328 (6133): 817-20

PMID: 2442615 · DOI:10.1038/328817a0

Transforming growth factor-alpha (TGF-alpha) is a polypeptide which is structurally related to epidermal growth factor (EGF) and binds to the EGF receptor. TGF-alpha synthesis occurs in a variety of neoplastic cells and during early fetal development but has not been reported in normal cells of the adult organisms. TGF-alpha has therefore been regarded as an embryonic growth factor which is inappropriately expressed during neoplasia. Here we report that primary cultures of normal human keratinocytes synthesize TGF-alpha. Furthermore, we show that addition of EGF or TGF-alpha to these cultures induces TGF-alpha gene expression, suggesting that a mechanism of auto-induction exists. Analysis of normal skin biopsies using in situ hybridization and immunohistochemistry demonstrates the in vivo presence of TGF-alpha messenger RNA and protein in the stratified epidermis.

MeSH Terms (14)

Cells, Cultured Epidermal Growth Factor Epidermis Gene Expression Regulation Growth Substances Humans Keratins Melanocytes Neoplasm Proteins Nucleic Acid Hybridization Peptide Biosynthesis Peptides RNA, Messenger Transforming Growth Factors

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