Differential effects of eicosapentaenoic acid and docosahexaenoic acid in promoting the differentiation of 3T3-L1 preadipocytes.

Murali G, Desouza CV, Clevenger ME, Ramalingam R, Saraswathi V
Prostaglandins Leukot Essent Fatty Acids. 2014 90 (1): 13-21

PMID: 24332315 · DOI:10.1016/j.plefa.2013.10.002

The objective of this study was to determine the effects of enrichment with n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), on the differentiation of 3T3-L1 preadipocytes. Enrichment with DHA but not EPA significantly increased the differentiation markers compared to control differentiated cells. DHA compared to EPA treatment led to a greater increase in adiponectin secretion and, conditioned media collected from DHA treated cells inhibited monocyte migration. Moreover, DHA treatment resulted in inhibition of pro-inflammatory signaling pathways. DHA treated cells predominantly accumulated DHA in phospholipids whereas EPA treatment led to accumulation of both EPA and its elongation product docosapentaenoic acid (DPA), an n-3 fatty acid. Of note, adding DPA to DHA inhibited DHA-induced differentiation. The differential effects of EPA and DHA on preadipocyte differentiation may be due, in part, to differences in their intracellular modification which could impact the type of n-3 fatty acids incorporated into the cells.

© 2013 Published by Elsevier Ltd.

MeSH Terms (7)

3T3-L1 Cells Adipocytes Animals Cell Differentiation Docosahexaenoic Acids Eicosapentaenoic Acid Mice

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