Intracellular neutralization of a virus using a cell-penetrating molecular transporter.

Sapparapu G, Sims AL, Aiyegbo MS, Shaikh FY, Harth EM, Crowe JE
Nanomedicine (Lond). 2014 9 (11): 1613-24

PMID: 24195674 · DOI:10.2217/nnm.13.139

AIMS - Antibodies are the principal mediator of immunity against reinfection with viruses. Antibodies typically neutralize viruses by binding to virion particles in solution prior to attachment to susceptible cells. Once viruses enter cells, conventional antibodies cannot inhibit virus infection or replication. It is desirable to develop an efficient and nontoxic method for the introduction of virus-inhibiting antibodies into cells.

MATERIALS & METHODS - In this article, we report a new method for the delivery of small recombinant antibody fragments into virus-infected cells using a dendrimer-based molecular transporter.

RESULTS & CONCLUSION - The construct penetrated virus-infected cells efficiently and inhibited virus replication. This method provides a novel approach for the immediate delivery of inhibitory antibodies directed to virus proteins that are exposed only in the intracellular environment. This approach circumvents the current and rather complicated expression of inhibitory antibodies in cells following gene transfer.

MeSH Terms (23)

Animals Antibodies Antibodies, Monoclonal Biological Transport Capsid Proteins Cytoplasm Dendrimers Enzyme-Linked Immunosorbent Assay Gene Transfer Techniques HIV-1 Humans Immunoglobulin Fragments Kidney Macaca mulatta Magnetic Resonance Spectroscopy Microscopy, Confocal Nanomedicine Neutralization Tests Peptides RNA, Small Interfering Rotavirus Virion Viruses

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