Structure and expression of a novel compact myelin protein - small VCP-interacting protein (SVIP).

Wu J, Peng D, Voehler M, Sanders CR, Li J
Biochem Biophys Res Commun. 2013 440 (1): 173-8

PMID: 24055875 · PMCID: PMC3828079 · DOI:10.1016/j.bbrc.2013.09.056

SVIP (small p97/VCP-interacting protein) was initially identified as one of many cofactors regulating the valosin containing protein (VCP), an AAA+ ATPase involved in endoplasmic-reticulum-associated protein degradation (ERAD). Our previous study showed that SVIP is expressed exclusively in the nervous system. In the present study, SVIP and VCP were seen to be co-localized in neuronal cell bodies. Interestingly, we also observed that SVIP co-localizes with myelin basic protein (MBP) in compact myelin, where VCP was absent. Furthermore, using nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopic measurements, we determined that SVIP is an intrinsically disordered protein (IDP). However, upon binding to the surface of membranes containing a net negative charge, the helical content of SVIP increases dramatically. These findings provide structural insight into interactions between SVIP and myelin membranes.

Published by Elsevier Inc.

MeSH Terms (16)

Amino Acid Sequence Animals Anions Carrier Proteins DNA, Complementary Escherichia coli Gene Expression Humans Membrane Proteins Mice Molecular Sequence Data Nuclear Proteins Plasmids Protein Structure, Secondary Rats Sciatic Nerve

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