Enzymes involved in the assimilation of one-carbon units by Pseudomonas MS.

Wagner C, Levitch ME
J Bacteriol. 1975 122 (3): 905-10

PMID: 238950 · PMCID: PMC246141 · DOI:10.1128/JB.122.3.905-910.1975

Pseudomonas MS can grow on methylamine and a number of other compounds containing C1 units as a sole source of carbon and energy. Assimilation of carbon into cell material occurs via the "serine pathway" since enzymes of this pathway are induced after growth on methylamine, but not malate or acetate. A mutant has been isolated which is unable to grow on methylamine or any other related substrate providing C1 units. This mutant is also unable to grow on acetate. Measurment of enzyme activities in cell-free extracts of wild-type cells showed that growth on methylamine caused induction of isocitrate lyase, a key enzyme in the glyoxylate cycle. The mutant organism lacks malate lyase, a key enzyme of the serine pathway, and isocitrate lyase as well. These results suggest that utilization of C1 units by Pseudomonas MS results in the net accumulation of acetate which is then assimilated into cell material via the glyoxylate cycle.

MeSH Terms (20)

Acetates Alcohol Oxidoreductases Carbon Cell-Free System Glycine Hydroxymethyltransferase Hydrogen-Ion Concentration Isocitrates Lyases Malates Methylamines Methylnitronitrosoguanidine Models, Biological Mutagens Mutation Oxo-Acid-Lyases Phosphoenolpyruvate Carboxykinase (GTP) Pseudomonas Pyruvates Soil Microbiology Transferases

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