Use of the noninvasive entero-test in the detection of Helicobacter pylori in children in an endemic area in Colombia.

Arboleda RN, Schneider BG, Bravo LE, Romero-Gallo J, Peek RM, Mera RM, Yepez MC, Campo C, Correa P
J Pediatr Gastroenterol Nutr. 2013 57 (2): 192-6

PMID: 23880626 · PMCID: PMC3725653 · DOI:10.1097/MPG.0b013e318293e1e1

BACKGROUND AND OBJECTIVE - Gastric infection with Helicobacter pylori (H pylori), a strong risk factor for gastric cancer, is highly prevalent in children residing in the Colombian Andes. We aimed to validate the use of the Entero-test to culture and genotype H pylori strains from asymptomatic Colombian children.

METHODS - Children (ages 10-15 years, n = 110, 80 of which were H pylori positive by the urea breath test [UBT]) were subjected to the Entero-test, and strings were cultured and/or used for DNA extraction for polymerase chain reaction (PCR). These children had been treated for H pylori in 2007. A second population of children (ages 10-15 years, n = 95),which had not been previously treated, was also subjected to the Entero-test.

RESULTS - Of UBT-positive children in the treated group, 29 of 80 (36%) Entero-test samples were H pylori culture positive; 29 additional string extracts were tested by PCR for the H pylori virulence factors cagA and vacA. PCR from cultures and extracts yielded a sensitivity of 74% and specificity of 87%. In the untreated group, 16 of 94 UBT-positive children (17%) produced Entero-tests that were culture positive. Fifty-eight of 94 (62%) string extracts were PCR positive for cagA and/or vacA. In previously treated children, H pylori strains were more often the less virulent vacA s2 (P = 0.001), m2 (P = 0.006), and i2 genotypes (P = 0.039).

CONCLUSIONS - The Entero-test may be used as a noninvasive test to detect H pylori in asymptomatic children residing in high-risk areas for gastric cancer. Treatment of H pylori in children was associated with less virulent genotypes.

MeSH Terms (19)

Antigens, Bacterial Bacterial Proteins Bacterial Typing Techniques Breath Tests Child Colombia DNA, Bacterial Endemic Diseases Female Genotype Helicobacter Infections Helicobacter pylori Humans Male Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity Stomach Neoplasms Virulence Factors

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