In vitro induction of regulatory CD4+CD8α+ T cells by TGF-β, IL-7 and IFN-γ.

Van Kaer L, Rabacal WA, Scott Algood HM, Parekh VV, Olivares-Villagómez D
PLoS One. 2013 8 (7): e67821

PMID: 23844100 · PMCID: PMC3701067 · DOI:10.1371/journal.pone.0067821

In vitro CD4(+) T cell differentiation systems have made important contributions to understanding the mechanisms underlying the differentiation of naive CD4(+) T cells into effector cells with distinct biological functions. Mature CD4(+) T cells expressing CD8αα homodimers are primarily found in the intestinal mucosa of men and mice, and to a lesser extent in other tissues such as peripheral blood. Although CD4(+)CD8α(+) T cells are easily identified, very little is known about their development and immunological functions. It has been reported, however, that CD4(+)CD8α(+) T cells possess regulatory properties. In this report, we present a novel in vitro differentiation system where CD4(+) T cells are stimulated to become CD4(+)CD8α(+) T cells in the presence of TGF-β, IL-7 and IFN-γ, resulting in cells with very similar features as CD4(+)CD8α(+) intraepithelial lymphocytes. This novel in vitro differentiation culture should provide a powerful and tractable tool for dissecting the differentiation and biological functions of CD4(+)CD8α(+) T cells.

MeSH Terms (22)

Adoptive Transfer Animals Antigens, CD Antigens, Differentiation CD4 Antigens CD8 Antigens Cell Differentiation Cell Lineage Core Binding Factor Alpha 3 Subunit CTLA-4 Antigen Cytokines Immunophenotyping Interferon-gamma Interleukin-7 Intestinal Mucosa Mice Mice, Knockout NK Cell Lectin-Like Receptor Subfamily K Programmed Cell Death 1 Receptor T-Lymphocytes, Regulatory Transforming Growth Factor beta Vitamin D

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