Pancreas-specific Cre driver lines and considerations for their prudent use.

Magnuson MA, Osipovich AB
Cell Metab. 2013 18 (1): 9-20

PMID: 23823474 · PMCID: PMC3732107 · DOI:10.1016/j.cmet.2013.06.011

Cre/LoxP has broad utility for studying the function, development, and oncogenic transformation of pancreatic cells in mice. Here we provide an overview of the Cre driver lines that are available for such studies. We discuss how variegated expression, transgene silencing, and recombination in undesired cell types have conspired to limit the performance of these lines, sometimes leading to serious experimental concerns. We also discuss preferred strategies for achieving high-fidelity driver lines and remind investigators of the continuing need for caution when interpreting results obtained from any Cre/LoxP-based experiment performed in mice.

Copyright © 2013 Elsevier Inc. All rights reserved.

MeSH Terms (15)

Animals DNA Nucleotidyltransferases Extracellular Matrix Proteins Integrases Mice Mice, Knockout Mice, Transgenic Models, Animal Pancreas Pancreatic Hormones Protein-Lysine 6-Oxidase Rats Rats, Transgenic Recombination, Genetic Transgenes

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