Maternal-fetal transfer of selenium in the mouse.

Burk RF, Olson GE, Hill KE, Winfrey VP, Motley AK, Kurokawa S
FASEB J. 2013 27 (8): 3249-56

PMID: 23651543 · PMCID: PMC3714584 · DOI:10.1096/fj.13-231852

Selenoprotein P (Sepp1) is taken up by receptor-mediated endocytosis for its selenium. The other extracellular selenoprotein, glutathione peroxidase-3 (Gpx3), has not been shown to transport selenium. Mice with genetic alterations of Sepp1, the Sepp1 receptors apolipoprotein E receptor-2 (apoER2) and megalin, and Gpx3 were used to investigate maternal-fetal selenium transfer. Immunocytochemistry (ICC) showed receptor-independent uptake of Sepp1 and Gpx3 in the same vesicles of d-13 visceral yolk sac cells, suggesting uptake by pinocytosis. ICC also showed apoER2-mediated uptake of maternal Sepp1 in the d-18 placenta. Thus, two selenoprotein-dependent maternal-fetal selenium transfer mechanisms were identified. Selenium was quantified in d-18 fetuses with the mechanisms disrupted. Maternal Sepp1 deletion, which lowers maternal whole-body selenium, decreased fetal selenium under selenium-adequate conditions but deletion of fetal apoER2 did not. Fetal apoER2 deletion did decrease fetal selenium, by 51%, under selenium-deficient conditions, verifying function of the placental Sepp1-apoER2 mechanism. Maternal Gpx3 deletion decreased fetal selenium, by 13%, but only under selenium-deficient conditions. These findings indicate that the selenoprotein uptake mechanisms ensure selenium transfer to the fetus under selenium-deficient conditions. The failure of their disruptions (apoER2 deletion, Gpx3 deletion) to affect fetal selenium under selenium-adequate conditions indicates the existence of an additional maternal-fetal selenium transfer mechanism.

MeSH Terms (19)

Animals Biological Transport Female Glutathione Peroxidase Immunohistochemistry LDL-Receptor Related Proteins Low Density Lipoprotein Receptor-Related Protein-2 Male Maternal-Fetal Exchange Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Fluorescence Placenta Pregnancy Selenium Selenoprotein P Time Factors Yolk Sac

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