Atropisomeric dihydroanthracenones as inhibitors of multiresistant Staphylococcus aureus.

Bara R, Zerfass I, Aly AH, Goldbach-Gecke H, Raghavan V, Sass P, Mándi A, Wray V, Polavarapu PL, Pretsch A, Lin W, Kurtán T, Debbab A, Brötz-Oesterhelt H, Proksch P
J Med Chem. 2013 56 (8): 3257-72

PMID: 23534483 · DOI:10.1021/jm301816a

Two bisdihydroanthracenone atropodiastereomeric pairs, including homodimeric flavomannin A (1) and the previously unreported flavomannin B (2), two new unsymmetrical dimers (3 and 4), and two new mixed dihydroanthracenone/anthraquinone dimers (5 and 6) were isolated from Talaromyces wortmannii , an endophyte of Aloe vera . The structures of 2-6 were elucidated by extensive NMR and mass spectrometric analyses. The axial chirality of the biaryls was determined using TDDFT ECD and VCD calculations, the combination of which however did not allow the assignment of the central chirality elements of 1. The compounds exhibited antibacterial activity against Staphylococcus aureus , including (multi)drug-resistant clinical isolates. Reporter gene analyses indicated induction of the SOS response for some of the derivatives, suggesting interference with DNA structure or metabolism. Fluorescence microscopy demonstrated defective segregation of the bacterial chromosome and DNA degradation. Notably, the compounds showed no cytotoxic activity, encouraging their further evaluation as potential starting points for antibacterial drug development.

MeSH Terms (17)

Aloe Animals Anthracenes Anti-Bacterial Agents BALB 3T3 Cells Cell Line, Tumor DNA, Bacterial Drug Resistance, Multiple, Bacterial Endophytes Eurotiales Humans Mice Microbial Sensitivity Tests Nuclear Magnetic Resonance, Biomolecular SOS Response, Genetics Staphylococcus aureus Stereoisomerism

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