Mapping and analysis of phosphorylation sites: a quick guide for cell biologists.

Dephoure N, Gould KL, Gygi SP, Kellogg DR
Mol Biol Cell. 2013 24 (5): 535-42

PMID: 23447708 · PMCID: PMC3583658 · DOI:10.1091/mbc.E12-09-0677

A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.

MeSH Terms (9)

Amino Acids Amino Acid Sequence Chromosome Mapping Databases, Protein Peptide Fragments Phosphoproteins Phosphorylation Signal Transduction Tandem Mass Spectrometry

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