UW solution improved with high anti-apoptotic activity by S-nitrosated human serum albumin.

Ishima Y, Shinagawa T, Yoneshige S, Kragh-Hansen U, Ohya Y, Inomata Y, Kai T, Otagiri M, Maruyama T
Nitric Oxide. 2013 30: 36-42

PMID: 23402724 · DOI:10.1016/j.niox.2013.01.004

S-Nitrosated human serum albumin (SNO-HSA) is useful in preventing liver ischemia/reperfusion injury, and SNO-HSA should thus be able to prevent cell injury during liver transplantation. However, the potential protective effect of SNO-HSA on a combination of cold and warm ischemia, which is obligatory when performing liver transplantation, has not been examined. Therefore, we evaluated the protective effect of SNO-HSA added to University of Wisconsin (UW) solution during cold or/and warm ischemia in situ and in vitro. First, we observed that apoptotic and necrotic cell death were increased during cold and warm ischemia, respectively. SNO-HSA, which possesses anti-apoptosis activity at low NO concentrations, can inhibit cold ischemia injury both in situ and in vitro. In contrast, SNO-HSA had no significant effect on warm liver ischemia injury which, however, can be reduced by UW solution. We also demonstrated that the cellular uptake of NO from SNO-HSA can occur during cold ischemia resulting in induction of heme oxygenase-1 within 3h of cold ischemia. Our results indicate that treatment with SNO-HSA or UW solution alone is not sufficient to inhibit liver injury during a period of both cold and warm ischemia. However, a combination of SNO-HSA and UW solution can be used to prevent the two types of ischemia. SNO-HSA-added UW solution could be very useful in transplantation, because the previously imposed constraints on preservation time can be removed. This is a great advantage in a situation as the present one with increased utilization of scarce donor organs for more recipients.

Copyright © 2013 Elsevier Inc. All rights reserved.

MeSH Terms (23)

Adenosine Allopurinol Analysis of Variance Animals Apoptosis Glutathione Hep G2 Cells Humans Insulin Liver Liver Diseases Liver Transplantation Male Necrosis Nitric Oxide Donors Nitroso Compounds Organ Preservation Solutions Raffinose Rats Rats, Wistar Reperfusion Injury Serum Albumin Serum Albumin, Human

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