Matrix-assisted laser desorption ionization imaging mass spectrometry: in situ molecular mapping.

Angel PM, Caprioli RM
Biochemistry. 2013 52 (22): 3818-28

PMID: 23259809 · PMCID: PMC3864574 · DOI:10.1021/bi301519p

Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the tissue that are subsequently recorded in a mass spectrometer. IMS is distinguished by the ability to directly measure molecules in situ ranging from small metabolites to proteins, reporting hundreds to thousands of expression patterns from a single imaging experiment. This article reviews recent advances in IMS technology, applications, and experimental strategies that allow it to significantly aid in the discovery and understanding of molecular processes in biological and clinical samples.

MeSH Terms (8)

Animals Breast Neoplasms Embryo, Mammalian Fixatives Lung Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tissue Array Analysis

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