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genetic determinants of intrinsic colistin tolerance in Acinetobacter baumannii.

Hood MI, Becker KW, Roux CM, Dunman PM, Skaar EP
Infect Immun. 2013 81 (2): 542-51

PMID: 23230287 · PMCID: PMC3553813 · DOI:10.1128/IAI.00704-12

Acinetobacter baumannii is a leading cause of multidrug-resistant infections worldwide. This organism poses a particular challenge due to its ability to acquire resistance to new antibiotics through adaptation or mutation. This study was undertaken to determine the mechanisms governing the adaptability of A. baumannii to the antibiotic colistin. Screening of a transposon mutant library identified over 30 genes involved in inducible colistin resistance in A. baumannii. One of the genes identified was lpsB, which encodes a glycosyltransferase involved in lipopolysaccharide (LPS) synthesis. We demonstrate that loss of LpsB function results in increased sensitivity to both colistin and cationic antimicrobial peptides of the innate immune system. Moreover, LpsB is critical for pathogenesis in a pulmonary model of infection. Taken together, these data define bacterial processes required for intrinsic colistin tolerance in A. baumannii and underscore the importance of outer membrane structure in both antibiotic resistance and the pathogenesis of A. baumannii.

MeSH Terms (20)

Acinetobacter baumannii Acinetobacter Infections Animals Anti-Bacterial Agents Antimicrobial Cationic Peptides Bacterial Proteins Colistin Drug Resistance, Multiple, Bacterial Female Glycosyltransferases Immune Tolerance Immunity, Innate Lipopolysaccharides Lung Mannosyltransferases Mice Mice, Inbred C57BL Molecular Sequence Data Mutation Pneumonia

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