In vitro cultivation of the hymenoptera genetic model, Nasonia.

Brucker RM, Bordenstein SR
PLoS One. 2012 7 (12): e51269

PMID: 23227258 · PMCID: PMC3515518 · DOI:10.1371/journal.pone.0051269

The wasp genus Nasonia is a genetic model with unique advantages for the study of interspecific differences, including haplodiploidy and interfertile species. However, as a parasitoid, Nasonia is confined within a fly host, thus restricting direct observations and manipulation of development over time. Here, we present the first in vitro cultivation method for this system that decouples Nasonia from its host, allowing continuous observations from embryo to adulthood. Using transwell plates and a simple Nasonia rearing medium, we demonstrate a technique that will significantly expand the utility of the Nasonia model.

MeSH Terms (9)

Animals Digestive System Physiological Phenomena Larva Models, Animal Models, Genetic Peristalsis Tissue Culture Techniques Wasps Wasp Venoms

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