Recent advances in the structural mechanisms of DNA glycosylases.

Brooks SC, Adhikary S, Rubinson EH, Eichman BF
Biochim Biophys Acta. 2013 1834 (1): 247-71

PMID: 23076011 · PMCID: PMC3530658 · DOI:10.1016/j.bbapap.2012.10.005

DNA glycosylases safeguard the genome by locating and excising a diverse array of aberrant nucleobases created from oxidation, alkylation, and deamination of DNA. Since the discovery 28years ago that these enzymes employ a base flipping mechanism to trap their substrates, six different protein architectures have been identified to perform the same basic task. Work over the past several years has unraveled details for how the various DNA glycosylases survey DNA, detect damage within the duplex, select for the correct modification, and catalyze base excision. Here, we provide a broad overview of these latest advances in glycosylase mechanisms gleaned from structural enzymology, highlighting features common to all glycosylases as well as key differences that define their particular substrate specificities.

Copyright © 2012 Elsevier B.V. All rights reserved.

MeSH Terms (7)

Animals DNA DNA Damage DNA Glycosylases Humans Protein Structure, Tertiary Structure-Activity Relationship

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