Monitoring the inflammatory response to infection through the integration of MALDI IMS and MRI.

Attia AS, Schroeder KA, Seeley EH, Wilson KJ, Hammer ND, Colvin DC, Manier ML, Nicklay JJ, Rose KL, Gore JC, Caprioli RM, Skaar EP
Cell Host Microbe. 2012 11 (6): 664-73

PMID: 22704626 · PMCID: PMC3377982 · DOI:10.1016/j.chom.2012.04.018

Systemic bacterial infection is characterized by a robust whole-organism inflammatory response. Analysis of the immune response to infection involves technologies that typically focus on single organ systems and lack spatial information. Additionally, the analysis of individual inflammatory proteins requires antibodies specific to the protein of interest, limiting the panel of proteins that can be analyzed. Herein we describe the application of matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) to mice systemically infected with Staphylococcus aureus to identify inflammatory protein masses that respond to infection throughout an entire infected animal. Integrating the resolution afforded by magnetic resonance imaging (MRI) with the sensitivity of MALDI IMS provides three-dimensional spatially resolved information regarding the distribution of innate immune proteins during systemic infection, allowing comparisons to in vivo structural information and soft-tissue contrast via MRI. Thus, integrating MALDI IMS with MRI provides a systems-biology approach to study inflammation during infection.

Copyright © 2012 Elsevier Inc. All rights reserved.

MeSH Terms (12)

Animals Female Imaging, Three-Dimensional Inflammation Magnetic Resonance Imaging Mice Mice, Inbred BALB C Pathology Sepsis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Staphylococcus aureus Whole Body Imaging

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