Generation of myometrium-specific Bmal1 knockout mice for parturition analysis.

Ratajczak CK, Asada M, Allen GC, McMahon DG, Muglia LM, Smith D, Bhattacharyya S, Muglia LJ
Reprod Fertil Dev. 2012 24 (5): 759-67

PMID: 22697126 · DOI:10.1071/RD11164

Human and rodent studies indicate a role for circadian rhythmicity and associated clock gene expression in supporting normal parturition. The importance of clock gene expression in tissues besides the suprachiasmatic nucleus is emerging. Here, a Bmal1 conditional knockout mouse line and a novel Cre transgenic mouse line were used to examine the role of myometrial Bmal1 in parturition. Ninety-two percent (22/24) of control females but only 64% (14/22) of females with disrupted myometrial Bmal1 completed parturition during the expected time window of 5p.m. on Day 19 through to 9a.m. on Day 19.5 of gestation. However, neither serum progesterone levels nor uterine transcript expression of the contractile-associated proteins Connexin43 and Oxytocin receptor differed between females with disrupted myometrial Bmal1 and controls during late gestation. The data indicate a role for myometrial Bmal1 in maintaining normal time of day of parturition.

MeSH Terms (14)

Animals ARNTL Transcription Factors Biological Clocks Female Gene Transfer Techniques Male Mice Mice, Inbred C57BL Mice, Knockout Myometrium Organ Specificity Parturition Pregnancy Time Factors

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