High-throughput antibody sequencing reveals genetic evidence of global regulation of the naïve and memory repertoires that extends across individuals.

Briney BS, Willis JR, McKinney BA, Crowe JE
Genes Immun. 2012 13 (6): 469-73

PMID: 22622198 · DOI:10.1038/gene.2012.20

Vast diversity in the antibody repertoire is a key component of the adaptive immune response. This diversity is generated centrally through the assembly of variable, diversity and joining gene segments, and peripherally by somatic hypermutation and class-switch recombination. The peripheral diversification process is thought to only occur in response to antigenic stimulus, producing antigen-selected memory B cells. Surprisingly, analyses of the variable, diversity and joining gene segments have revealed that the naïve and memory subsets are composed of similar proportions of these elements. Lacking, however, is a more detailed study, analyzing the repertoires of naïve and memory subsets at the level of the complete V(D)J recombinant. This report presents a thorough examination of V(D)J recombinants in the human peripheral blood repertoire, revealing surprisingly large repertoire differences between circulating B-cell subsets and providing genetic evidence for global control of repertoire diversity in naïve and memory circulating B-cell subsets.

MeSH Terms (9)

Adult Antibody Diversity B-Lymphocyte Subsets High-Throughput Nucleotide Sequencing Humans Immunoglobulin G Immunoglobulin M Immunologic Memory V(D)J Recombination

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