A flow cytometry-based dopamine transporter binding assay using antagonist-conjugated quantum dots.

Kovtun O, Ross EJ, Tomlinson ID, Rosenthal SJ
Chem Commun (Camb). 2012 48 (44): 5428-30

PMID: 22543630 · DOI:10.1039/c2cc31951a

Here we present the development and validation of a flow cytometry-based dopamine transporter (DAT) binding assay that uses antagonist-conjugated quantum dots (QDs). We anticipate that our QD-based assay is of immediate value to the high throughput screening of novel DAT modulators.

MeSH Terms (16)

Binding, Competitive Biotin Dopamine Dopamine Antagonists Dopamine Plasma Membrane Transport Proteins Flow Cytometry Fluorescent Dyes Gene Expression Regulation HEK293 Cells High-Throughput Screening Assays Humans Phorbol Esters Piperazines Polyethylene Glycols Quantum Dots Spectrometry, Fluorescence

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