Utility of immunohistochemical staining with FLI1, D2-40, CD31, and CD34 in the diagnosis of acquired immunodeficiency syndrome-related and non-acquired immunodeficiency syndrome-related Kaposi sarcoma.

Rosado FG, Itani DM, Coffin CM, Cates JM
Arch Pathol Lab Med. 2012 136 (3): 301-4

PMID: 22372906 · DOI:10.5858/arpa.2011-0213-OA

CONTEXT - Kaposi sarcoma (KS) is a vascular tumor frequently associated with advanced human immunodeficiency virus infection, advanced age, or iatrogenic immunosuppression. Immunohistochemistry for CD31 and CD34, and more recently for FLI1 and D2-40, has been used as ancillary diagnostic tests for KS, despite little information regarding the sensitivities and differential staining patterns of the latter 2 markers in the major clinical subtypes and histologic stages of KS.

OBJECTIVE - This retrospective study aims to assess the prevalence of the vascular markers D2-40 and FLI1 in the main clinical subgroups and tumor stages of KS.

DESIGN - Twenty-four cases of KS (12 acquired immunodeficiency syndrome [AIDS]-related cases and 12 non-AIDS-related cases; 11 nodular-stage and 13 patch/plaque-stage KS) were stained for CD34, CD31, D2-40, and FLI1 by immunohistochemistry. The distribution of immunoreactivity was compared between the clinical subtypes and tumor stages of KS using the Mann-Whitney test.

RESULTS - CD31, CD34, D2-40, and FLI1 strongly and diffusely stained tumor cells in 75%, 92%, 67%, and 92% of AIDS-related cases and 58%, 92%, 67%, and 75% of non-AIDS-related cases, respectively. Differences in the proportions of positive cases between AIDS-related and non-AIDS-related cases did not reach statistical significance. No significant staining differences were observed between nodular- and patch/plaque-stage KS either.

CONCLUSIONS - There are no differences in the distribution of immunohistochemical reactivity for CD31, CD34, D2-40, or FLI1 between AIDS-related and non-AIDS-related KS or between nodular- and patch/plaque-stage KS. All of the markers studied demonstrated high sensitivity in both clinical settings and both stages of tumor progression.

MeSH Terms (16)

Acquired Immunodeficiency Syndrome Antibodies, Monoclonal, Murine-Derived Antigens, CD34 Biomarkers, Tumor Diagnosis, Differential Disease Progression Humans Immunohistochemistry Neoplasm Staging Platelet Endothelial Cell Adhesion Molecule-1 Proto-Oncogene Protein c-fli-1 Reproducibility of Results Retrospective Studies Sarcoma, Kaposi Sensitivity and Specificity Staining and Labeling

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