Blockade of prostaglandin E2 signaling through EP1 and EP3 receptors attenuates Flt3L-dependent dendritic cell development from hematopoietic progenitor cells.

Singh P, Hoggatt J, Hu P, Speth JM, Fukuda S, Breyer RM, Pelus LM
Blood. 2012 119 (7): 1671-82

PMID: 22110249 · PMCID: PMC3286345 · DOI:10.1182/blood-2011-03-342428

Dendritic cell (DC) homeostasis, like all mature blood cells, is maintained via hierarchal generation from hematopoietic precursors; however, little is known about the regulatory mechanisms governing DC generation. Here, we show that prostaglandin E(2) (PGE(2)) is required for optimal Flt3 ligand-mediated DC development and regulates expression of the Flt3 receptor on DC-committed progenitor cells. Inhibition of PGE(2) biosynthesis reduces Flt3-mediated activation of STAT3 and expression of the antiapoptotic protein survivin, resulting in increased apoptosis of DC-committed progenitor cells. Reduced DC development caused by diminished PGE(2) signaling is reversed by overexpression of Flt3 or survivin in DC progenitors and conversely is mimicked by STAT3 inhibition. PGE(2) regulation of DC generation is specifically mediated through the EP1 and EP3 G protein PGE(2) receptors. These studies define a novel DC progenitor regulatory pathway in which PGE(2) signaling through EP1/EP3 receptors regulates Flt3 expression and downstream STAT3 activation and survivin expression, required for optimal DC progenitor survival and DC development in vivo.

MeSH Terms (19)

Animals Cell Differentiation Cells, Cultured Dendritic Cells Dinoprostone Hematopoietic Stem Cells Hormone Antagonists Humans Infant, Newborn Inhibitor of Apoptosis Proteins Membrane Proteins Mice Mice, Inbred C57BL Mice, Knockout Receptors, Prostaglandin E, EP1 Subtype Receptors, Prostaglandin E, EP3 Subtype Signal Transduction STAT3 Transcription Factor Survivin

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