Cloning of the Schizosaccharomyces pombe TFIID gene reveals a strong conservation of functional domains present in Saccharomyces cerevisiae TFIID.

Hoffmann A, Horikoshi M, Wang CK, Schroeder S, Weil PA, Roeder RG
Genes Dev. 1990 4 (7): 1141-8

PMID: 2210373 · DOI:10.1101/gad.4.7.1141

The gene encoding the Schizosaccharomyces pombe TATA box-binding factor (TFIID) was cloned and sequenced. The gene contains three introns and codes for a polypeptide of 231 amino acids. The cDNA-expressed protein showed both TATA box-binding and basal transcription activities. The carboxy-terminal three-quarters of S. pombe TFIID shares an extraordinary degree of amino acid sequence homology with a corresponding region of Saccharomyces cerevisiae TFIID that has been shown to be necessary and sufficient for TATA box-binding and basal transcription activities. In contrast, the amino-terminal regions of the S. pombe and S. cerevisiae TFIIDs differ markedly in amino acid sequence and composition. Structure and function relationships of TFIID are discussed in light of these data.

MeSH Terms (16)

Amino Acid Sequence Base Sequence DNA Fungal Proteins Genes, Fungal Genes, Regulator Molecular Sequence Data Phylogeny Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae Schizosaccharomyces Sequence Homology, Nucleic Acid Species Specificity Structure-Activity Relationship Transcription Factors Transcription Factor TFIID

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