Berberine protects against high fat diet-induced dysfunction in muscle mitochondria by inducing SIRT1-dependent mitochondrial biogenesis.

Gomes AP, Duarte FV, Nunes P, Hubbard BP, Teodoro JS, Varela AT, Jones JG, Sinclair DA, Palmeira CM, Rolo AP
Biochim Biophys Acta. 2012 1822 (2): 185-95

PMID: 22027215 · PMCID: PMC3366688 · DOI:10.1016/j.bbadis.2011.10.008

Berberine (BBR) has recently been shown to improve insulin sensitivity in rodent models of insulin resistance. Although this effect was explained partly through an observed activation of AMP-activated protein kinase (AMPK), the upstream and downstream mediators of this phenotype were not explored. Here, we show that BBR supplementation reverts mitochondrial dysfunction induced by High Fat Diet (HFD) and hyperglycemia in skeletal muscle, in part due to an increase in mitochondrial biogenesis. Furthermore, we observe that the prevention of mitochondrial dysfunction by BBR, the increase in mitochondrial biogenesis, as well as BBR-induced AMPK activation, are blocked in cells in which SIRT1 has been knocked-down. Taken together, these data reveal an important role for SIRT1 and mitochondrial biogenesis in the preventive effects of BBR on diet-induced insulin resistance.

Copyright © 2011 Elsevier B.V. All rights reserved.

MeSH Terms (22)

AMP-Activated Protein Kinases Animals Berberine Cell Line Diet, High-Fat Glucose Hormones Hyperglycemia Insulin Resistance Male Mice Mitochondria Mitochondria, Muscle Muscle, Skeletal Myoblasts NAD Obesity Organelle Biogenesis Phosphorylation Rats Rats, Sprague-Dawley Sirtuin 1

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