Synthesis, characterization of calcium phosphates/polyurethane composites for weight-bearing implants.

Yoshii T, Dumas JE, Okawa A, Spengler DM, Guelcher SA
J Biomed Mater Res B Appl Biomater. 2012 100 (1): 32-40

PMID: 21953899 · DOI:10.1002/jbm.b.31917

Calcium phosphate (CaP)/polymer composites have been studied as an alternative graft material for the treatment of bone defects. In this study, lysine-triisocyanate-based polyurethane (PUR) composites were synthesized from both hydroxyapatite (HA) and β-tricalcium phosphate (TCP) to reduce the brittleness of CaP and increase the bioactivity of the polymer. The mechanical properties and in vitro cellular response were investigated for both HA/PUR and TCP/PUR composites. The composites were implanted in femoral defects in rats, and in vivo bioactivity was evaluated by X-rays, micro-computed tomography (μCT), and histological sections. In biomechanical testing, PUR improved the mechanical properties of the CaP, thus rendering it potentially suitable for weight-bearing applications. In vitro cell culture studies showed that CaP/PUR composites are biocompatible, with β-TCP enhancing the cell viability and proliferation relative to HA. CaP/PUR composites also supported the differentiation of osteoblastic cells on the materials. When implanted in rat femoral defects, the CaP/PUR composites were biocompatible and osteoconductive with no adverse inflammatory response, as evidenced by X-rays, μCT images, and histological sections. Additionally, a histological examination showed evidence of cellular infiltration and appositional remodeling. These results suggest that CaP/PUR composites could be potentially useful biomaterials for weight-bearing orthopaedic implants.

Copyright © 2011 Wiley Periodicals, Inc.

MeSH Terms (13)

Animals Bone Substitutes Calcium Phosphates Cell Line Durapatite Femur Male Materials Testing Mice Polyurethanes Rats Rats, Sprague-Dawley X-Ray Microtomography

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